United States Ryder Cup captain Davis Love III has named Rickie Fowler, Matt Kuchar and JB Holmes as three of his four wildcard picks.
This year’s event is being held in Hazeltine, Minnesota from 30 September to 2 October.
Europe, captained this time by Darren Clarke, have won the past three competitions.
Last month, Clarke named Lee Westwood, Martin Kaymer and rookie Thomas Pieters as his three wildcards.
Fowler, 27, has competed in two Ryder Cups before but has failed to win any of his eight matches.
Kuchar, 38, won bronze in the Rio Olympics and is competing in his fourth Ryder Cup.
Holmes, 34, has only played in one Ryder Cup previously, helping the US to victory in 2008.
Love III, who also captained the US when they lost in 2012, will name the final member of his 12-man team on 25 September after the Tour Championship finishes.
Dustin Johnson, Jordan Spieth, Phil Mickelson, Patrick Reed, Jimmy Walker, Brooks Koepka, Brandt Snedeker and Zach Johnson all qualified automatically.
BBC Sport’s golf correspondent Iain Carter:
“On paper it is a big call to leave out the world number seven, but Bubba Watson’s lack of recent form and failure to win a point at Gleneagles [in 2014] counted against him.
“The selection of Kuchar makes total sense, given his experience and last month’s Olympic bronze medal. Holmes was one of the stars the last time the US beat Europe back in 2008 and Fowler is a world-class talent, playing his first home Ryder Cup. He has yet to win a match in two previous contests and will be under pressure to deliver.”